Search results for " isotopes"

showing 10 items of 460 documents

Heavy enzymes and the rational redesign of protein catalysts

2019

Abstract An unsolved mystery in biology concerns the link between enzyme catalysis and protein motions. Comparison between isotopically labelled “heavy” dihydrofolate reductases and their natural‐abundance counterparts has suggested that the coupling of protein motions to the chemistry of the catalysed reaction is minimised in the case of hydride transfer. In alcohol dehydrogenases, unnatural, bulky substrates that induce additional electrostatic rearrangements of the active site enhance coupled motions. This finding could provide a new route to engineering enzymes with altered substrate specificity, because amino acid residues responsible for dynamic coupling with a given substrate present…

010402 general chemistryProtein Engineering01 natural sciencesBiochemistryCatalysisEnzyme catalysisisotope effectsCatalytic DomainDihydrofolate reductaseMolecular BiologyAlcohol dehydrogenasechemistry.chemical_classificationalcohol dehydrogenasesCarbon Isotopesdihydrofolate reductasesbiologyBacteriaNitrogen Isotopes010405 organic chemistryConceptOrganic ChemistryAlcohol DehydrogenaseActive siteSubstrate (chemistry)Protein engineeringDeuteriumCombinatorial chemistrymolecular dynamics0104 chemical sciencesKineticsTetrahydrofolate Dehydrogenaseenzyme engineeringEnzymechemistrybiology.proteinBiocatalysisMolecular MedicineConcepts
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Environmental and biological factors are joint drivers of mercury biomagnification in subarctic lake food webs along a climate and productivity gradi…

2021

Subarctic lakes are getting warmer and more productive due to the joint effects of climate change and intensive land-use practices (e.g. forest clear-cutting and peatland ditching), processes that potentially increase leaching of peat- and soil-stored mercury into lake ecosystems. We sampled biotic communities from primary producers (algae) to top consumers (piscivorous fish), in 19 subarctic lakes situated on a latitudinal (69.0-66.5 degrees N), climatic (+3.2 degrees C temperature and +30% precipitation from north to south) and catchment land-use (pristine to intensive forestry areas) gradient. We first tested how the joint effects of climate and productivity influence mercury biomagnific…

010504 meteorology & atmospheric sciencesBiomagnificationTROPHIC POSITIONmaankäyttö010501 environmental sciencesMETHYLMERCURY01 natural sciencesFood chainBiological FactorsONTARIO LAKESCHAIN STRUCTUREClimate changeympäristömyrkytWaste Management and DisposalLand-useApex predatorTrophic levelkalatStable isotopes2. Zero hungerFRESH-WATEREcologyFishesvesiekosysteemitBIOACCUMULATIONselkärangattomatPollutionSubarctic climateclimate changeProductivity (ecology)Environmental MonitoringFood chain lengthEnvironmental EngineeringFood Chainelohopeachemistry.chemical_elementstable isotopeskasautuminenWHITEFISHland-useEnvironmental ChemistryAnimalsravintoketjutEcosystem1172 Environmental sciences0105 earth and related environmental sciencesfishfood chain lengthLake ecosystemMercury15. Life on landilmastonmuutoksetCHARR SALVELINUS-ALPINUSinvertebratesInvertebratesMercury (element)LakesFishchemistryisotooppianalyysi13. Climate actionEnvironmental scienceMARINEWater Pollutants Chemical
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Changements environnementaux survenant à la limite Oligocène/Miocène du bassin des Limagnes (Massif central, France).

2018

16 pages; International audience; Continental environments are very sensitive to climatic variations. A unique opportunity to study the climate changes around the Oligocene/Miocene boundary is offered by the Limagne graben Basin (France) where this stage boundary is well constrained by fossils. Indeed, some localities of the Limagne Graben Basin are so rich in mammal remains that they have become a European reference for mammal biostratigraphy. The dominant sedimentary facies of the lacustrine deposits in the northern part of the Limagne Graben Basin are composed of poorly cemented marls and calcarenites containing various plants and animals remains (e.g. algae, fish bones and teeth, gastro…

010504 meteorology & atmospheric sciencesContext (language use)Biostratigraphy010502 geochemistry & geophysics01 natural sciencesPaleontology[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistrycarbonates lacustresMarl14. Life underwaterstromatolitebassin des Limagnes0105 earth and related environmental sciencesPalynologygeographygeography.geographical_feature_categoryLimagne Basinisotopes de l’oxygènebiologylimite oligo-miocèneoxygen isotopeslcsh:QE1-996.5GeologyMassif15. Life on landbiology.organism_classificationlacustrine carbonateGrabenlcsh:GeologyStromatolite13. Climate action[SDU.STU.ST]Sciences of the Universe [physics]/Earth Sciences/StratigraphyapatitePeriod (geology)Oligocene/Miocene boundarybiozoneGeologyBSGF - Earth Sciences Bulletin
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Interpretation of the nitrogen isotopic composition of Precambrian sedimentary rocks: Assumptions and perspectives

2016

International audience; Nitrogen isotope compositions in sedimentary rocks (d(15)N(sed)) are routinely used for reconstructing Cenozoic N-biogeochemical cycling and are also being increasingly applied to understanding the evolution of ancient environments. Here we review the existing knowledge and rationale behind the use of d(15)N(sed) as a proxy for the Precambrian N-biogeochemical cycle with the aims of (i) identifying the major uncertainties that affect analyses and interpretation of nitrogen isotopes in ancient sedimentary rocks, (ii) developing a framework for interpreting the Precambrian d(15)N(sed) record, (iii) testing this framework against a database of Precambrian d(15)N(sed) va…

010504 meteorology & atmospheric sciencesEarth scienceNitrogen isotopesMetamorphismGeologyNitrogen biogeochemical cycle010502 geochemistry & geophysicsEarly Earth01 natural sciencesIsotopes of nitrogenDiagenesisPaleontologyPrecambrianGeologic time scale13. Climate actionGeochemistry and Petrology[SDU]Sciences of the Universe [physics]Ocean oxygenationSedimentary rock14. Life underwaterPrecambrianCenozoicGeology[ SDU ] Sciences of the Universe [physics]0105 earth and related environmental sciences
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Soil development on sediments and evaporites of the Messinian crisis

2020

Abstract Vast areas in the Mediterranean are characterised by evaporite deposits of the Messinian crises (c. 6–5.3 Ma BP). During this period, large deposits were built up in shallow lagoon-like systems and are now found in southern Italy, Albania, Cyprus and Turkey. So far, soil formation on evaporites has been studied predominantly in subarid to arid environments. Although the formation of soils has received new significance, little is known about the evolutional trajectories on evaporites of the Mediterranean. We therefore studied soil formation in the Caltanissetta basin (Sicily) where evaporites are most widespread. The lithologies included the sequence: marine clay deposits, laminated…

010504 meteorology & atmospheric sciencesEvaporite1904 Earth-Surface ProcessesGeochemistryEarthWeathering04 agricultural and veterinary sciences01 natural sciencesDiagenesis10122 Institute of GeographyPedogenesisSurface ProcessesSettore AGR/14 - PedologiaSoil formation Evaporites Clay mineralogy Weathering Diatomite Stable isotopes CarbonatesLeaching (pedology)Marl040103 agronomy & agriculture0401 agriculture forestry and fisheriesKaoliniteAridisol910 Geography & travelGeology0105 earth and related environmental sciencesEarth-Surface Processes
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Impact of basin burial and exhumation on Jurassic carbonates diagenesis on both sides of a thick clay barrier (Paris Basin, NE France).

2014

27 pages; International audience; Several diagenetic models have been proposed for Middle and Upper Jurassic carbonates of the eastern Paris Basin. The paragenetic sequences are compared in both aquifers to propose a diagenetic model for the Middle and Late Jurassic deposits as a whole. Petrographic (optical and cathodoluminescence microscopy), structural (fracture orientations) and geochemical (δ18O, δ13C, REE) studies were conducted to characterize diagenetic cements, with a focus on blocky calcite cements, and their connection with fracturation events. Four generations of blocky calcite (Cal1-Cal4) are identified. Cal1 and Cal2 are widespread in the dominantly grain-supported facies of t…

010504 meteorology & atmospheric sciencesEvaporiteCarbonateStratigraphyGeochemistryJurassic[ SDU.STU.ST ] Sciences of the Universe [physics]/Earth Sciences/Stratigraphy010502 geochemistry & geophysicsOceanography01 natural sciencesDiagenesisPetrographyOxygen and carbon isotopesPaleontologychemistry.chemical_compoundParis Basin[ SDU.STU.MI ] Sciences of the Universe [physics]/Earth Sciences/MineralogyRare earth elements0105 earth and related environmental sciencesCalciteGeologyCementation (geology)6. Clean waterCretaceousDiagenesisGeophysicschemistry[SDU.STU.ST]Sciences of the Universe [physics]/Earth Sciences/StratigraphyFaciesEconomic GeologyPaleogeneGeology[SDU.STU.MI]Sciences of the Universe [physics]/Earth Sciences/Mineralogy
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Understanding volcanoes in the Vanuatu arc

2016

We report the first helium isotope survey of volcanic gases, hot springs and some olivine phenocrysts along the Vanuatu island arc, from Tanna in the south to Vanua Lava in the north. Low CO2 content and low He-3/He-4 ratios in thermal fluids of Epi (4.0 +/- 0.1 R-a), Efate (4.5 +/- 0.1 R-a) and Pentecost (5.3 +/- 0.5 R-a) islands coherently indicate reduced mantle gas leakage and crustal contamination by radiogenic helium on these extinct volcanic systems of the former (Pliocene) arc. Instead, presently active Vanuatu volcanoes display He-3/He-4 and C/He-3 ratios typical of subduction-related volcanic arcs: He-3/He-4 ratios range from 6.4 +/- 0.5 Ra in southernmost Tanna and 7.23 +/- 0.09 …

010504 meteorology & atmospheric sciencesLavaEarth scienceGeochemistryVanuatu arcHelium isotopes[SDU.STU]Sciences of the Universe [physics]/Earth Sciences010502 geochemistry & geophysics01 natural sciencesVolcanic fluidsVolcanic GasesGeochemistry and Petrologyevent0105 earth and related environmental sciencesBasaltevent.disaster_typegeographygeography.geographical_feature_categoryVolcanic arcHotspot contributionFumaroleExtinct and active volcanoesMantle sourceSettore GEO/08 - Geochimica E VulcanologiaGeophysicsVolcano13. Climate actionVanuatu arc Volcanic fluids Helium isotopes Extinct and active volcanoes Mantle source Hotspot contributionIsland arcPhenocrystGeology
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The imprint of subduction fluids on subducted MORB-derived melts (Sierra del Convento Mélange, Cuba)

2011

International audience; Major and trace element signatures and Sr-Nd-Pb isotope data for muscovite (Ms)-bearing amphibolite blocks and associated muscovite-bearing trondhjemite and quartz-muscovite rocks from the Sierra del Convento melange (eastern Cuba) indicate that Proto-Caribbean oceanic crust underwent wet partial melting processes during Mesozoic subduction and after accretion to the upper plate. Trace element normalized patterns of Ms-bearing amphibolites are enriched in light rare earth elements (LREE) and large-ion lithophile elements (LILE) and evidence variable trace element transfer from the Proto-Caribbean subducting slab to the mantle wedge. Ms-bearing trondhjemites show LREE…

010504 meteorology & atmospheric sciencesMantle wedge[SDE.MCG]Environmental Sciences/Global ChangesFluid compositionTrondhjemiteGeochemistrySedimentary imprint010502 geochemistry & geophysics01 natural sciences[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/GeochemistryGeochemistry and PetrologyOceanic crust0105 earth and related environmental sciencesBasaltTrace elementsSubductionbiologyPartial meltingTrace elementGeologySr-Nd-Pb isotopesbiology.organism_classification13. Climate actionAdakiteLileGeologySlab meltsLithos
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Abiotic and biotic controls on methane formation down to 2.5 km depth within the Precambrian Fennoscandian Shield

2017

Abstract Despite a geological history characterised by high temperature and pressure processes and organic carbon deprived crystalline bedrock, large amounts of hydrocarbons are found in deep groundwaters within Precambrian continental shields. In many sites, methane comprises more that 80% of the dissolved gas phase reaching concentrations of tens of mmol l −1 . In this study, we used isotopic methods to study the carbon isotope systematics and sources of crustal methane within the Fennoscandian Shield. The main study sites were the Outokumpu Deep Drill Hole and the Pyhasalmi mine in Finland, both of which allow groundwater sampling down to 2.5 km depth and have been previously studied for…

010504 meteorology & atmospheric sciencesMethanogenesista1171GeochemistryMineralogychemistry.chemical_element010502 geochemistry & geophysicsmetaani01 natural sciencesMethanechemistry.chemical_compoundPrecambrianPyhäsalmikalsiittiGeochemistry and PetrologyNatural gasgrafiitti0105 earth and related environmental sciencesTotal organic carbongraphitebusiness.industrymethane15. Life on landethaneOutokumpuchemistryvetycarbon isotopes13. Climate actionIsotopes of carbonhydrogenFennoscandian shieldbusinesscalciteCarbonGroundwaterGeologyGeochimica et Cosmochimica Acta
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Geodynamic control on carbonate diagenesis: Petrographic and isotopic investigation of the Upper Jurassic formations of the Paris Basin (France).

2007

23 pages; International audience; The Oxfordian and Kimmeridgian carbonates of the eastern edge of the Paris Basin display poor reservoir properties (Φb15% and Kb0.1 md). The petrographic investigation and the resultant paragenetic sequence show that this is due to extensive precipitation of 2 types of almost synchronous blocky LMC (Low Magnesian Calcite) cements during mesogenesis (burial). Stable isotope investigation of these non-recrystallized cements, through both microdrilling sampling and in-situ SIMS (Secondary IonMass Spectrometry) analysis, shows that they originated from slightly buffered meteoric fluids (−3‰SMOW to −8‰SMOW) flowing through the Malm carbonate aquifers of the east…

010504 meteorology & atmospheric sciencesStratigraphy[SDE.MCG]Environmental Sciences/Global ChangesCarbonates010502 geochemistry & geophysics[ SDU.STU.ST ] Sciences of the Universe [physics]/Earth Sciences/StratigraphyBasin evolution01 natural sciencesUnconformityDiagenesisPetrographyPaleontologychemistry.chemical_compoundUnconformities[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/GeochemistryLower Cretaceous0105 earth and related environmental sciencesStable isotopesgeographygeography.geographical_feature_categoryGeologyMassif[ SDU.STU.GC ] Sciences of the Universe [physics]/Earth Sciences/GeochemistryCretaceousDiagenesis[ SDE.MCG ] Environmental Sciences/Global ChangesDenudationchemistry[SDU.STU.ST]Sciences of the Universe [physics]/Earth Sciences/StratigraphyMeteoric waterCarbonateGeology
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